Abstract: This paper proposes a mechanical model that can be used to analyze the deformation of the cell during indentation experiments based on the membrane theory. The mechanical model of the cell is validated through the macro-indentation tests on latex balls and PVC balls, the theoretical analysis and the comparison of the indentation testing curves of the different cells. At the same time, a specific mechanical analysis of the single chondrocytes is carried out by using the new model, and the mechanical parameters of the cell and the stress distribution on the surface of the cell membrane are obtained. A new idea is thus provided for the study of the mechanical properties of single cells.